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Organization of genes encoding membrane proteins of the Escherichia coli ferrienterobactin permease
Author(s) -
Chenault S. S.,
Earhart C. F.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb00787.x
Subject(s) - operon , biology , escherichia coli , complementation , open reading frame , gene , permease , genetics , enterobactin , membrane transport protein , nucleic acid sequence , transposon mutagenesis , gene product , repressor , peptide sequence , transposable element , transporter , gene expression , phenotype , genome
Summary Transposon mutagenesis and plasmid complementation studies have identified two genes, fepD and fepG , which are essential for ferrienterobactin transport in Escherichia coli. These genes mapped In the enterobactin gene cluster and genetic evidence indicated that they are transcribed as part of an operon (fepD, fepG, fepC). The nucleotide sequence of fepD was determined; it could encode a hydrophobic 33.8kDa protein with sequence homologies to other iron and vitamin B12 transport proteins. Also identified, between fepD and fepB , was an open reading frame (ORF43) with no detectable function; its 43 kDa protein product (P43) was seen on polyacrylamide gels. The fepD–C operon and ORF43 were divergently transcribed from a 110bp region containing a binding site for the repressor protein Fur.