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Isolation and properties of the RepA1 protein of the IncFII replicon, RepFIC
Author(s) -
Maas R.,
Oppenheim J.,
Saadi S.,
Fuchs T.,
Maas W. K.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb00767.x
Subject(s) - replicon , biology , open reading frame , plasmid , genetics , coding region , computational biology , gene , peptide sequence
Summary The initiator protein RepA1 of the IncFII replicon RepFIC derived from the enterotoxin plasmid EntP307 has been cloned under the control of the λ P l promoter. This has enabled us to overproduce this protein and study its properties. Here we show that RepA1 is a soluble basic protein with an experimentally determined molecular weight of 40000. Deletion analysis indicates that the overproduced protein originates from the open reading frame which we previously designated as coding for RepA1. We have also shown that the replication function of the replicon RepFIC depends on the intact RepA1 coding frame.