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A suppressor of yeast spp81/ded1 mutations encodes a very similar putative ATP‐dependent RNA helicase
Author(s) -
Jamieson D. J.,
Beggs J. D.
Publication year - 1991
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1991.tb00753.x
Subject(s) - biology , rna helicase a , helicase , dead box , genetics , yeast , suppressor , mutation , rna , gene , microbiology and biotechnology
Summary The spp81/ded1 mutations were isolated as suppressors of a Saccharomyces cerevisiae pre‐mRNA splicing mutation, prp 8‐1, The SPP81/DED1 gene encodes a isolated plasmids which were able to suppress the cold‐sensitive growth defect of SPP81 mutants. These plasmids encoded a gene (named DBP 1) which mapped to chromosome XVI and not to the SPP 81/DED1 locus on chromosome XV. The cloned gene suppressed the defect of SPP 81/ ded 1 mutants when present on both high and low copy‐number plasmids but complemented SPP 81/ ded 1 null mutants only when present on high copy‐number plasmids. In contrast to the SPP 81/ DED 1 gene the DBP 1 gene was not essential for cell viability. The nucleotide sequence of the DBP 1 gene revealed that it also encoded a putative ATP‐dependent RNA helicase which showed considerable similarity at the amino acid level to the SPP81/DED1 protein.