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Characterization of a Mycobacterium tuberculosis insertion sequence belonging to the IS3 family
Author(s) -
McAdam R. A.,
Hermans P. W. M.,
Soolingen D.,
Zainuddin Z. F.,
Catty D.,
Embden J. D. A.,
Dale J. W.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb02073.x
Subject(s) - insertion sequence , biology , orfs , transposable element , genetics , frameshift mutation , mycobacterium tuberculosis , inverted repeat , sequence (biology) , transposon mutagenesis , plasmid , mutagenesis , open reading frame , mutation , tuberculosis , dna , gene , peptide sequence , genome , medicine , pathology
Summary A repetitive element (IS986), previously isolated from Mycobacterium tuberculosis and shown to detect multiple restriction fragment‐length polymorphisms (RFLPs), has been sequenced. It consists of a potential insertion sequence of 1358bp, with 30‐bp inverted repeat ends. IS986 has four potentially significant open reading frames (ORFs): ORFa1, ORFa2 and ORFb on one strand and ORFc on the complementary strand. The sequences of the potential translated products identify IS986 as a member of the IS3 family, with an apparent frameshift between ORFa1 and ORFa2. IS986 has potential as a highly specific probe for detection and typing of M. tuberculosis , as well as for transposon mutagenesis of mycobacteria. The sequence of IS986 is virtually identical to that of another recently described element, IS6110 (Thierry et al., 1990).