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Characterization of two DNA populations of Giardia lamblia
Author(s) -
OrtegaPierres G.,
Alonso R.,
Cervantes L.,
Montanez C.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb02048.x
Subject(s) - biology , giardia lamblia , giardia , dna , genetics , computational biology , microbiology and biotechnology , evolutionary biology
Summary Total DNA was isolated from the parasitic protozoan Giardia lamblia and separated into two distinct populations of different densities by centrifugation through CsCI gradients containing Hoechst dye 33258. The two populations obtained were characterized by restriction enzyme analysis and nucleic acid hybridization. The less‐dense population contains non‐repetitive DNA and may encode mainly structural genes, such as those for α‐ and β‐tubulin. Digestion of the DNA with several restriction endonucleases showed that the denser band was composed of a 5.5 kb unit which contains the C. lamblia ribosomal RNA cistron in tandem repeated organization.