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Molecular evolution of class A β‐lactamases: phylogeny and patterns of sequence conservation
Author(s) -
Pastor N.,
Piñero D.,
Valdés A. M.,
Soberón X.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb02045.x
Subject(s) - biology , genetics , phylogenetics , conserved sequence , multiple sequence alignment , sequence alignment , molecular evolution , nucleic acid sequence , computational biology , peptide sequence , gene , evolutionary biology
Summary We present a multiple alignment of the amino acid sequences of eight class A β‐lactamases and utilized it to propose a phylogeny, based on the nucleotide sequences of their corresponding genes. We have also used the alignment, together with the α‐carbon co‐ordinates of the Staphylococcus aureus protein, to search systematically for neighbouring residues that share the same pattern of conservation among the different members of the protein family. The distribution of invariant residues and of groups of residues with co‐ordinate changes map, predominantly, at the region of the active site and at interfaces between structural elements, respectively. We have also contrasted the distribution of conserved residues with the positions which are known to differ in mutants and variants of class A β‐lactamases.