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Identification and characterization of a porcine‐specific transferrin receptor in Actinobacillus pleuropneumoniae
Author(s) -
Gonzalez G. C.,
Caamano D. L.,
Schryvers A. S.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00692.x
Subject(s) - transferrin , actinobacillus pleuropneumoniae , biology , biotinylation , transferrin receptor , agarose , biotin , serotype , microbiology and biotechnology , bacterial outer membrane , biochemistry , escherichia coli , gene
Summary All six strains of Actinobacillus pleuropneumoniae screened for the ability to use different transferrins as a source of iron for growth were capable of using porcine but not human, bovine, or avian transferrins. A specific binding activity for porcine transferrin (pTf) was expressed in cells grown in the presence of specific iron‐chelators and was repressed by addition of excess iron. Two iron‐repressible outer‐membrane proteins of 105 and 56kD were specifically isolated from serotype 1, 2 and 7 strains of A. pleuropneumoniae by an affinity‐isolation method using biotinylated porcine transferrin and streptavidin‐agarose.