Premium
Streptococcus pneumoniae possesses canonical Escherichia coli (sigma 70) promoters
Author(s) -
Morrison D. A.,
Jaurin B.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00689.x
Subject(s) - biology , promoter , genetics , escherichia coli , primer extension , gene , streptococcus pneumoniae , dna , base pair , microbiology and biotechnology , gene expression , bacteria , base sequence
Summary Summary Seventeen DNA fragments from Streptococcus pneumoniae were randomly cloned in Escherichia coli with selection for promoter activity. The fragments were sequenced and the promoter locations were determined by primer extension analysis. Examination for sites similar to the E. coli major consensus promoter sequence revealed such a site in each of the seventeen fragments, located five to eight base pairs upstream of the point at which transcription was initiated in the E. coli host. Thus, the abundance of promoter activity found in pneumococcal DNA cloned in E coli hosts arises primarily from sigma‐70‐type promoter structures. Combined with the observation that such sequences are usually found just upstream of, but not within, pneumococcal genes, this implies that one class (perhaps the major class) of pneumococcal promoters closely resembles the canonical E. coli promoter consensus.