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Mycoplasma pneumoniae DNA gyrase genes
Author(s) -
Colman S. D.,
Hu P.C.,
Bott K. F.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00687.x
Subject(s) - biology , dna gyrase , gene , genetics , mycoplasma pneumoniae , dna , mycoplasmataceae , mycoplasma , mollicutes , microbiology and biotechnology , computational biology , escherichia coli , pneumonia , history , archaeology
Summary We have identified a clone from a λEMBL3 library containing a 19kb insert of Mycoplasma pneumoniae DNA which includes the genes that encode both subunits of DNA gyrase. The gyrB gene and the 5’end of the gyrA gene have been subcloned into M13. The gryB gene is 1953bp in length and overlaps the gryA gene by a single base. The nucleotide sequence of these subclones has significant homology to previously reported gyrase genes. In terms of the size of gyrB gene and its proximity to the gyrA gene, M. pneumoniae is more similar to Bacillus subtilis than to Escherichia coli.