Premium
Integration host factor affects expression of two genes at the conjugal transfer origin of plasmid R100
Author(s) -
Dempsey W. B.,
Fee B. E.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00674.x
Subject(s) - biology , plasmid , gene , host (biology) , genetics , expression (computer science) , host factor , horizontal gene transfer , genome , computer science , programming language
Summary Integration host factor (IHF) binds to two sites near the origin of transfer of the conjugative antibiotic resistance plasmid, R100. DNase I footprinting shows that one site is immediately adjacent to or/T and the gene X promoter, and another is adjacent to the traM promoter. A third site, known only from retardation gels, is near the traJ promoter. The relative promoter activities of genes X, traJ and traM are reduced in himA mutants (IHF), as measured by chloramphenicol‐resistance assays. Transcript analyses by Northern blots showed a reduction in size of the principal gene X and traJ transcripts in the absence of IHF.