Premium
Novobiocin‐resistance sequences from the novobiocin‐producing strain Streptomyces niveus
Author(s) -
Mitchell J. I.,
Logan P. G.,
Cushing K. E.,
Ritchie D. A.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00655.x
Subject(s) - novobiocin , biology , streptomyces , streptomycetaceae , streptomyces albus , actinomycetales , genetics , microbiology and biotechnology , molecular cloning , gene , bacteria , peptide sequence
Summary Two distinct DNA sequences expressing novobiocin resistance In Streptomyces lividans were cloned from the novobiocin‐producing species Streptomyces niveus. Clone pGL 101 (5kb) conferred resistance to 510μg ml −1 novobiocin, whereas clones pGL 102 and pGL 103, which carry the same 6.5kb insert but in opposite orientations, expressed resistance to 150μg ml −1 . The cloned inserts from pGL 101 and pGL 103 failed to hybridize with each other or with the cloned novobiocin‐resistant gyrB sequence from Streptomyces sphaeroides. Both probes hybridized strongly with DNA from the novobiocin‐producing species S. niveus and S. sphaeroides but no hybridization (pGL 103) or very weak hybridization (pGL 101) was detected with DNA from the non‐producing species S. lividans , Streptomyces griseus and Streptomyces antibioticus. S. niveus contains at least three novobiocin‐resistance determinants with the pGL 101 and pGL 103 cloned sequences specific for novobiocin‐producing strains of Streptomyces.