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The complete nucleotide sequence of the Pasteurella multocida toxin gene and evidence for a transcriptional repressor, TxaR
Author(s) -
Petersen S. K.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00652.x
Subject(s) - biology , repressor , pasteurella multocida , gene , operon , genetics , microbiology and biotechnology , nucleic acid sequence , homology (biology) , frameshift mutation , escherichia coli , mutation , gene expression , bacteria
Summary The osteolytic toxin of Pasteurella multocida induces bone resorption in vivo and in vitro (Foged et al. , 1988; Kimman et al. , 1987). In this report the toxin‐encoding toxA gene is sequenced, and the deduced primary structure of the toxin shows a protein of 1285 amino acids, containing a striking homology to a metal‐binding motif. Evidence that expression of the toxA gene is repressed at a transcriptional level in Escherichia coli is presented. Repression could be abolished either by deletion of a region upstream of toxA , or by a putative frame‐shift mutation in the same region. The repressor protein encoded within this region was efficient in trans , and was named TxaR.