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Mini‐Mu mediates deletion‐inversions in vivo by intra‐transposon transposition
Author(s) -
Leach D. R. F.,
Okely E. A.,
PercyRobb M. I.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00624.x
Subject(s) - transposable element , biology , transposition (logic) , bacteriophage mu , gene duplication , genetics , in vivo , transposase , inversion (geology) , transpose , microbiology and biotechnology , gene , physics , genome , paleontology , linguistics , philosophy , eigenvalues and eigenvectors , structural basin , quantum mechanics
Summary We have shown that a mini‐Mu can transpose into itself in vivo to generate a circle containing only transposon sequences. This deletion‐inversion product, which has previously been observed in vitro , is formed by non‐replicative transposition and has directly repeated Mu ends. It therefore cannot undergo further rounds of transposition and retains the two copies of the target sequence duplicated in the event. Thus we have been able to confirm that a mini‐Mu can undergo non‐replicative reactions in vivo and that these generate a 5bp target site duplication, as has been shown to occur following replicative transposition and lysogenization with Mu.