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Characterization of the repertoire of hypervariable regions in the Protein II (opa) gene family of Neisseria gonorrhoeae
Author(s) -
Connelly T. D.,
Shaffer D.,
Can J. G.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00610.x
Subject(s) - hypervariable region , biology , neisseria gonorrhoeae , genetics , gene , southern blot , sequence analysis , neisseria , microbiology and biotechnology , bacteria
Summary P.11 outer membrane proteins of Neisseria gonorrhoeae are encoded by a family of closely related genes. Although the genes are highly conserved, major differences in sequence among them occur in two short regions, designated hypervariable regions 1 (HV 1 ) and 2 (HV 2 ). In this study, we determined the number and DNA sequence of the hypervariable regions in the P.11 genes of strain FA1090. The FA1090 chromosome contained at least eleven P.11 loci, having six different versions each of HV 1 and HV 2 among them. Southern blotting with HV‐specific oligonucleotides showed that each version was present in one to three copies, and that there were nine unique combinations of HV 1 and HV 2 in the P.M genes. Although each of the versions of HV 1 or HV 2 had a unique DNA sequence, there were some similarities among them, particularly when certain ones were compared. Restriction fragments containing only the HV regions were cloned into an expression vector to demonstrate that the epitopes recognized by a set of monoclonal antibodies specific for different FA1090 P.11 proteins were completely encoded by either HV 1 or HV 2 .