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Escherichia coli cells resistant to the DNA gyrase inhibitor, ciprofloxacin, overproduce a 60 kD protein homologous to GroEL
Author(s) -
Hallett P.,
Mehlert A.,
Maxwell A.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00602.x
Subject(s) - biology , groel , dna gyrase , escherichia coli , microbiology and biotechnology , ciprofloxacin , homologous chromosome , bacterial protein , dna , escherichia coli proteins , homologous recombination , bacteria , genetics , gene , antibiotics
Summary Using a variety of mutagenic methods, we have generated a series of ciprofloxacin‐resistant mutants derived from Escherichia coli strains which overproduce the DNA gyrase A protein. Many of these mutants are found to overexpress a 60 kD protein which is shown to be highly homologous in terms of N ‐terminal a mi no acid sequence to the E. coli heat‐shock protein, GroEL. Other evidence confirms that the 60 kD protein is unrelated to DNA gyrase and is similar, but not identical, to GroEL.