Premium
Isolated DNA repeat region from fcrA76 , the Fc‐binding protein gene from an M‐type 76 strain of group A streptococci, encodes a protein with Fc‐binding activity
Author(s) -
Heath D. G.,
Boyle M. D. P.,
Cleary P. P.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00567.x
Subject(s) - biology , protein a/g , protein g , microbiology and biotechnology , binding protein , gene , protein a , plasmid , dna , escherichia coli , binding site , retinoblastoma like protein 1 , dna binding protein , recombinant dna , gene product , gene expression , biochemistry , antibody , genetics , fusion protein , transcription factor
Summary The DNA repeat region of fcrA76 , the gene encoding a group A streptococcal Fc‐binding protein, was sub‐cloned in‐frame into an Escherichia coli plasmid expression vector. The expressed protein product displayed the same Fc‐binding properties as the full‐length Fc‐binding protein expressed from fcrA76. The affinity‐purified, full‐length Fc‐binding protein was found to compete with staphylococcal protein A or streptococcal protein G for binding to beads coated with human IgG. These results are consistent with earlier studies suggesting that the binding sites on human IgG for protein A, protein G and the type II Fc‐binding protein from group A streptococci are located at the interface of the CH 2 and CH 3 domains of the Fc region.