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Transcriptional analysis of the glnB‐glnA region of Rhizobium leguminosarum biovar viciae
Author(s) -
Chiurazzi M.,
Laccarino M.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00550.x
Subject(s) - biology , rpon , promoter , rhizobium leguminosarum , gene , genetics , primer extension , sigma factor , nucleic acid sequence , transcription (linguistics) , microbiology and biotechnology , nucleotide , rhizobiaceae , gene expression , bacteria , symbiosis , linguistics , philosophy
Summary We report that the glnB and gln A genes of Rhizobium leguminosarum biovar viciae are preceded by promoters located upstream of each gene. We find the presence of a glnB–glnA and a glnA mRNA whose intracellular concentration changes two‐ to three‐fold when R leguminosarum is grown on different nitrogen sources. Primer extension analysis shows unique transcriptional initiation sites upstream of glnB and glnA. The glnB promoter is rpoN(ntrA) ‐dependent, while the glnA promoter does not contain a typical consensus sequence for previously described promoters. In Klebsiella pneumoniae the glnB promoter requires active ntrC and ntrA genes and a DNA fragment containing 53 nucleotides upstream of the transcription initiation site shows full promoter activity, thus indicating that no NtrC binding sites are necessary for this activation in the glnB upstream region.