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Phage‐conversion of cytotoxin production in Pseudomonas aeruginosa
Author(s) -
Hayashi T.,
Baba T.,
Matsumoto H.,
Terawaki Y.
Publication year - 1990
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1990.tb00547.x
Subject(s) - lysogen , biology , temperateness , pseudomonas aeruginosa , lysogenic cycle , dna , gene , genome , strain (injury) , microbiology and biotechnology , phagemid , bacteriophage , prophage , genetics , bacteria , escherichia coli , anatomy
Summary We isolated a temperate phage which carried the cytotoxin gene ( ctx ) from a cytotoxin (CTX)‐producing Pseudomonas aeruginosa strain, PA158. The phage, φCTX, had a head with a hexagonal outline and a contractile tail with tail fibres. The phage genome was a linear double‐stranded 35.5kb DNA with single‐stranded cohesive ends ( cos ). The attP, cos and ctx genes were all located very close to one another within a 2.3kb segment on the phage genome in the order given (in the circular form). φCTX converted CTX non‐producing P. aeruginosa strains into CTX producers. A single copy of φCTX DNA was integrated at the same site on the host chromosome ( attB ) in every lysogen, including PA15B. However, the amount of CTX produced in these lysogens varied from strain to strain and was less than that in PA158.