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Mutations that alter the helix‐turn‐helix region of the spollAC protein: a Bacillus subtilis sporulation‐specific sigma factor
Author(s) -
Yudkin M. D.,
Millonig J. H.,
Appleby L.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb01815.x
Subject(s) - bacillus subtilis , biology , helix (gastropod) , sigma factor , helix turn helix , turn (biochemistry) , sigma , genetics , spore , microbiology and biotechnology , dna binding protein , biochemistry , transcription factor , gene , escherichia coli , bacteria , physics , ecology , rna polymerase , snail , quantum mechanics
Summary It has previously been shown that spollAC561 , a mutation that diminishes the incidence of sporulation by more than six orders of magnitude, alters the residue at position 13 of the helix‐turn‐helix region of the sporulation‐specific sigma factor encoded by spoll AC from valine to methionine (Yudkin, 1987b). We have now found that four spontaneous revertants, which sporulate at an incidence of 30‐60%, all contain transitions within the codon that was altered by spo ‐561. The mutant methionine is replaced by isoleucine in two revertants, and by threonine in the other two.