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Characterization of a cis regulatory DNA element necessary for formate induction of the formate dehydrogenase gene ( fdhF ) of Escherichia coli
Author(s) -
Birkmann A.,
Bock A.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb01807.x
Subject(s) - biology , formate dehydrogenase , gene , formate , enhancer , promoter , regulatory sequence , transcription (linguistics) , genetics , mutant , dna , escherichia coli , microbiology and biotechnology , transcription factor , gene expression , biochemistry , linguistics , philosophy , catalysis
Summary The fdhF gene, encoding the selenopolypeptide of formate dehydrogenase (FDH H ). has a ‐12/‐24 nif ‐type consensus promoter. A cis ‐acting DNA element, which is required for the regulation of the promoter by formate under anaerobic conditions, has been identified. This regulatory sequence of about 25bp in length is located 110 bp upstream of the transcription start site. By analysing a variety of mutant constructs in this region (5’deletions, internal deletions and point mutations) we were able to identify a hexanucleotide sequence ‐GTCACG‐, which is important for the formate regulation of the fdhF promoter. The data also indicate that this element has many of the properties characteristic of eukaryotic enhancers.