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Expression of the recE gene during induction of the SOS response in Bacillus subtilis recombination‐deficient strains
Author(s) -
Gassel M.,
Alonso J. C.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00277.x
Subject(s) - biology , bacillus subtilis , recbcd , gene , psychological repression , microbiology and biotechnology , nalidixic acid , gene expression , mutation , exonuclease , reporter gene , regulation of gene expression , genetics , escherichia coli , bacteria , dna polymerase
Summary A transcriptional fusion of the recE gene to a reporter gene has been constructed. Expression of recE was found to be induced upon damage to DNA with either mitomycin C or nalidixic acid. This specific transcriptional induction is blocked by a recE mutation. Mutations affecting the recB, recF and recL gene products markedly reduced induction. However, de‐repression of recE seems to be independent of the ATP‐dependent DNase activity of the exonuclease V enzyme (also called AddAB enzyme).