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Cloning a genomic region required for a high‐affinity iron‐uptake system in Rhizobium meliloti 1021
Author(s) -
Gill P. R.,
Neilands J. B.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00268.x
Subject(s) - biology , transposable element , siderophore , mutant , genomic dna , genetics , rhizobium , cloning (programming) , sinorhizobium meliloti , positional cloning , gene , computer science , programming language
Summary A collection of transposon‐induced mutants of Rhizobium meliloti 1021 defective in siderophore‐mediated iron assimilation were obtained and classified as biosynthetic, transport or regulatory. Several of the mutations were cloned and the adjacent sequences were used to acquire complementing DNA from the wild type. A single genomic region of about 35kb complemented all of the mutants deficient in production of the siderophore.