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Molecular cloning and expression of a locus ( mdoA ) implicated in the biosynthesis of membrane‐derived oligosaccharides in Escherichia coli
Author(s) -
Lacroix J.M.,
Tempête M.,
Menichi B.,
Bohin J.P.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00267.x
Subject(s) - biology , escherichia coli , plasmid , biosynthesis , locus (genetics) , mutant , cloning (programming) , molecular cloning , gene , genetics , bacterial outer membrane , cloning vector , dna , microbiology and biotechnology , biochemistry , peptide sequence , computer science , programming language
Summary Mutants of Escherichia coli defective in the mdoA locus are blocked at an early stage in the biosynthesis of membrane‐derived oligosaccharides. The mdoA locus has now been cloned into multicopy plasmids. A 5kb DNA fragment is necessary to complement mdoA mutations. Cells harbouring the mdoA + plasmid produced three to four times more MDO than wild‐type cells. MDO overproduction did not affect the degree of MDO substitution with sn‐1‐phosphoglycerol residues. The biosynthesis of MDO remained under osmotic control in overproducing strains.