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Yeast 2μm vectors replicate and undergo recombination in Torulaspora delbrueckii
Author(s) -
Compagno C.,
Ranzi B. M.,
Martegani E.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00251.x
Subject(s) - torulaspora delbrueckii , biology , plasmid , saccharomyces cerevisiae , genetics , transformation (genetics) , replicate , yeast , recombinant dna , recombination , shuttle vector , gene , computational biology , vector (molecular biology) , saccharomyces , statistics , mathematics
Summary In order to develop a procedure for transformation of the industrial yeast Torulaspora delbrueckii , we have constructed a set of recombinant plasmids carrying Saccharomyces cerevisiae ARS and 2μm origin of replication and kanamycin‐G418 resistance gene of Tn903(601) as a selective marker. In this paper we show that S. cerevisiae ARS vectors can replicate autonomously and that vectors bearing the whole S. cerevisiae 2 μ sequence yield stable transform ants. We also present evidence to show that 2μm vectors undergo an FLP‐mediated inter‐ and intramolecular recombination, which suggests that T. delbrueckii can support the amplification and partition mechanisms of these plasmids.