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Analysis of the chromosomal location of two copies of a Bordetella pertussis insertion sequence
Author(s) -
McPheat W. L.,
Hanson J. H.,
Livey I.,
Robertson J. S.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00248.x
Subject(s) - bordetella pertussis , biology , insertion sequence , gene , adenylate kinase , genetics , adenylate cyclase toxin , sequence (biology) , cyclase , microbiology and biotechnology , insertion , nucleic acid sequence , transposable element , pertussis toxin , mutation , genome , g protein , bacteria , receptor
Summary IS481v1 and IS481v2 are two copies of a Bordetella pertussis insertion sequence element. We have shown that IS481V1 is located within 3kbp of the start of the adenylate cyclase gene whilst IS481v2 is immediately adjacent to the end of the agglutinogen 2 gene and provides the stop codon for that gene. In addition, IS481v1 and IS481v2 were present at these two specific sites in nine strains of B. pertussis , including two Phase IV strains which expressed neither adenylate cyclase nor agglutinogen 2 and three Phase I strains which did not express agglutinogen 2. The loss of expression in these strains is not the result of DNA rearrangements at the sites of IS481 v1 or IS481v2.