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Specificity of insertion of IS91 , an insertion sequence present in α‐haemolysin plasmids of Escherichia coli
Author(s) -
Mendiola M. V.,
Cruz F.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00247.x
Subject(s) - biology , plasmid , insertion sequence , sequence (biology) , dna , genetics , insert (composites) , escherichia coli , hemolysin , nucleic acid sequence , microbiology and biotechnology , gene , transposable element , genome , virulence , mechanical engineering , engineering
Summary We have determined the DNA sequences of eight different insertions of IS91 in a specifically engineered recipient plasmid of known DNA sequence (pSU300). The sequences at the termini of IS91 are 5′ ‐CGAG‐TAGG… CCTATCGAT. IS91 inserts specifically 5′ to either one of the tetranucleotides 5′‐GAAC or 5′‐CAAG, and always in the same relative orientation with respect to the sequence of the target. Except in one special case, no duplications of the recipient DNA were produced at the site of insertion.