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Expression of gonococcal protein II in Escherichia coli by translational fusion
Author(s) -
Palmer L.,
Brooks G. F.,
Falkow S.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00214.x
Subject(s) - biology , escherichia coli , fusion protein , microbiology and biotechnology , genetics , recombinant dna , gene
Summary Aprotein II (P.II) gene from Neisseria gonorrhoeae was cloned in Escherichia coli and characterized by DNA sequence analysis. As with other reported P.II sequences, this gene contains an ATG initiation codon which is out of frame with respect to the remainder of the P.II amino acid sequence. A translational fusion was constructed in E. coli which linked the P.II sequence to the signal peptide of β‐lactamase. This P.II fusion differs from the gonococcal protein only in the first seven residues at the N terminus. In E. coli , the P.II fusion product exhibits properties analogous to those of P.II in N. gonorrhoeae. The P.II fusion product is a major component of the E. coli outer membrane and it is exposed on the cell surface. The P.II fusion protein also exhibits the heat‐modifiable phenotype of gonococcal P.II.