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Topographical alterations in proteins I of Neisseria gonorrhoeae correlated with lipooligosaccharide variation
Author(s) -
Judd R. C.,
Shafer W. M.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00211.x
Subject(s) - antigenicity , neisseria gonorrhoeae , antigen , biology , immunogenicity , polyacrylamide gel electrophoresis , gel electrophoresis , blot , microbiology and biotechnology , strain (injury) , epitope , neisseria meningitidis , neisseria , bacteria , biochemistry , gene , genetics , enzyme , anatomy
Summary Four transformant strains of Neisseria gonorrhoeae were generated, two of which (WS3 and WS5) had protein I subclass A (P.IA) and two which (WS2 and WS4) had protein I subclass B (P.IB). Analysis of the strains demonstrated that the two P.IA‐bearing strains differed in lipooligosaccharide (LOS) and H.8 antigen, as assessed by sodium dodecyl sulphate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and immuno‐blotting. The WS5 strain had slow‐migrating LOS and H.8 antigen, and the WS3 strain had fast‐migrating LOS and H.8 antigen. The P.IB‐bearing strains also had either slow‐migrating LOS and H.8 antigen (WS4) or fast‐migrating LOS and H.8 antigen (WS2). Structural and exposure analysis revealed that although the P.IAs were identical in the WS3 and WS5 strains, there was a slight alteration of the exposure of the proteins which correlated with altered LOS and/or H.8 antigen. The P.IBs were also shown to be structurally identical, but the LOS and/or H.8 antigen variation in these strains correlated with a more pronounced alteration in the exposure of the P.IB molecules. The differences in protein I (P.I) exposure were generally found in highly negatively charged regions of the molecule, suggesting that the immunogenicity and/or antigenicity of the P.I molecules may vary as a result of LOS and/or H.8 antigen alterations.