Premium
virG of Agrobacterium tumefaciens plasmid pTiC58 encodes a DNA‐binding protein
Author(s) -
Powell B. S.,
Rogowsky P. M.,
Kado C. I.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00186.x
Subject(s) - agrobacterium tumefaciens , biology , plasmid , ti plasmid , fusion protein , agrobacterium , microbiology and biotechnology , escherichia coli , gene , dna , expression vector , antiserum , genetics , recombinant dna , transformation (genetics) , antibody
Summary Virulence genes of the Agrobacterium tumefaciens Ti plasmid are positively regulated by the products of virA and virG. To study the DNA‐binding properties of the VirG protein, a translational fusion between virG and the frpEgene of Escherichia coli was constructed, and antiserum was raised against the encoded fusion protein. Using this antiserum, a protein of M r = 29000, a size similar to that calculated from the virG nucleotide sequence, was detected in an E. coli strain harbouring a virG expression vector. Both the VirG protein and the fusion protein were found, by filter‐binding and gel retardation analyses, to bind DNA nonspecifically. These data support an existing model for the two‐component regulatory systems of bacteria.