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Mitochondrial cytochrome b genes with a six‐nucleotide deletion or single‐nucleotide substitutions confer resistance to antimycin A in the yeast Kluyveromyces lactis
Author(s) -
Coria R.,
Garcfa M.,
Brunner A.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00145.x
Subject(s) - biology , mutant , kluyveromyces lactis , isoleucine , kluyveromyces , genetics , asparagine , biochemistry , nucleic acid sequence , nucleotide , antimycin a , gene , amino acid , leucine , saccharomyces cerevisiae , mitochondrion
Summary Extrachromosomal mutants resistant to antimycin, from the yeast Kluyveromyces lactis , have been isolated, genetically characterized, and assigned to two specific genetic loci (Brunner et al. , 1987). In the present work the cytochrome b nucleotide sequence from six of these mutants was determined. Five mutants had single point mutations, corresponding to transverslons. In one mutant, a six‐base‐pair deletion, beginning at nucleotide 689, was observed. The amino acid sequence derived from the coding strand showed that, in three independent antimycin‐resistant mutants, a change of asparagine 31 into lysine took place (two of these mutants are also resistant to diuron). Two other mutants showed a change from lysine 228 into isoleucine (or methionine). Leucine 230, isoleucine 231, and threonine 232, were lost in the deletion mutant and were replaced by serine.