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Structure and function of hot spots providing signals for site‐directed specific recombination and gene expression in Tn 21 transposons
Author(s) -
Schmidt F. R. J.,
Nucken E. J.,
Henschke R. B.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00140.x
Subject(s) - biology , transposable element , gene , genetics , recombination , homology (biology) , microbiology and biotechnology , mutant
Summary Tn 21‐ and Tn 3 ‐related transposons are widespread and carry various resistance determinants. The insertion points of different resistance genes were precisely defined in Tn 2424 , Tn 1696 , Tn 2410 , Tn 4000 and its derivatives and compared to the corresponding sites in Tn7, pSA, R388, R46, Tn 2603 , Tn 1331 and in Tn 3 ‐related elements. Insertional‘hot spots’located at the 3′ end of different genes comprised 55 nucleotides and yielded more than 90% homology to the corresponding consensus sequence, termed hs1. Elements of this class were found to direct recA‐ independent generation of deletions. Flanking the 5’ends, hs2 (CTAAAACAAAGTTA) comprised the terminal nucleotides of hs1. Functional properties of hot spots as recognition sites for site‐specific recombination and regulation of gene expression indicate that they might be involved in transfer, stable inheritance and expression of prokaryotic genes.