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Phosphoribosylpyrophosphate (PRPP)‐less mutants of Escherichia coli
Author(s) -
HoveJensen B.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00134.x
Subject(s) - biology , escherichia coli , plasmid , mutant , genetics , dna , gene , microbiology and biotechnology , histidine , biochemistry , amino acid
Summary A DNA fragment encoding kanamycin resistance was inserted in vitro into a plasmid‐borne prs gene encoding phosphoribosylpyrophosphate synthetase of Escherichia coli. The resulting plasmids were subsequently transferred to the chromosome by homologous recombination and the haploid strains prs ‐3::Kan R and prs‐4::Kan R were obtained. These strains were fully viable, but required guanosine, uridine, histidine, tryptophan and nicotinamide mononucleotide. There was no phosphoribosylpyrophosphate synthetase activity or phosphoribosylpyrophosphate pool in the mutant strains. These results show that phosphoribosytpyrophosphate synthetase is dispensable for E. coli.