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CRP/cAMP‐ and CytR‐regulated promoters in Escherichia coli K12: the cdd promoter
Author(s) -
ValentinHansen P.,
Holst B.,
Josephsen J.,
Hammer K.,
Albrechtsen B.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00120.x
Subject(s) - biology , repressor , promoter , camp receptor protein , transcription (linguistics) , transcription factor , genetics , microbiology and biotechnology , gene , gene expression , linguistics , philosophy
Summary Transcriptional regulation of the deoP 2 promoter by the cyclic AMP/cyclic AMP receptor protein complex (cAMP/CRP) and the CytR repressor requires two high‐affinity CRP targets located around ‐41 and ‐93 bp preceding the start site for transcription. Here we report the structure of cddP , another CRP/CytR‐regulated promoter. In common with what was found in deo , the cdd promoter also contains multiple CRP targets. Thus, using the DNasel footprinting procedure, tandem CRP binding sites were identified around ‐41 and ‐93. These findings support a general model for CytR binding and CytR regulation, in which (i) CytR and the CRP/cAMP complex bind to similar or Identical targets, (ii) two or more targets are necessary for proper binding of CytR to a promoter region, and (iii) CytR represses transcription by antagonizing cAMP/CRP activation.