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Cloning and characterization of NSP1 , a locus encoding a component of a CDC 25‐dependent, nutrient‐responsive pathway in Saccharomyces cerevisiae
Author(s) -
Tripp M. L.,
Bouchard R. A.,
Pi R.
Publication year - 1989
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1989.tb00113.x
Subject(s) - biology , phosphorylation , saccharomyces cerevisiae , dephosphorylation , cdc25 , phosphoprotein , mutant , locus (genetics) , microbiology and biotechnology , gene product , gene , biochemistry , cyclin dependent kinase 1 , phosphatase , cell cycle , gene expression
Summary The NSP 1 gene in Saccharomyces cerevisiae has been identified by its ability, when expressed at high levels, to bypass the CDC 25 requirement for growth. Sequence analysis of the cloned NSP 1 locus suggests that the NSP 1 product contains 269 amino acids and has a membrane‐spanning domain at its carboxyl terminus. The NSP 1 protein does not have sequence similarity to other known proteins, and is not related to the CDC 25 protein, or to any of the previously described suppressors of CDC 25 mutants. Phosphoprotein analysis of NSP 1‐suppressed cells indicates that the NSP 1 product controls the phosphorylation of two 31 kD proteins whose phosphorylation and de‐phosphorylation are strongly correlated with cell‐cycle arrest and proliferation, respectively, and suggests that the NSP 1 product is an important downstream element of a CDC 25‐dependent, nutrient‐responsive, phosphorylation pathway.