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Molecular cloning of a Salmonella typhi LT‐like enterotoxin gene
Author(s) -
Fernández M.,
SierraMadero J.,
Vega H.,
Vázquez M.,
LópezVidal Y.,
RuízPalacios G. M.,
Calva E.
Publication year - 1988
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1988.tb00094.x
Subject(s) - biology , salmonella typhi , enterotoxin , cloning (programming) , salmonella , gene , microbiology and biotechnology , molecular cloning , genetics , bacteria , escherichia coli , gene expression , computer science , programming language
Summary Diarrhoea is a common event during typhoid fever; nevertheless, the possible participation of a diarrhoea‐inducing enterotoxin has not been described (Roy et al. , 1985). Recombinant bacteriophage λ FDC1 was isolated from a genomic library of Salmonella typhi , the causal agent of typhoid fever, by screening with a probe for the B subunit gene of the heat‐labile, cholera‐like, Escherichia coli enterotoxin (LT). λ FDC1 codes for an enterotoxin that causes secretion in rat ileal loops, that elongates Chinese hamster ovary (CHO) cells, that is recognized by antibodies against LT, and does not bind in vitroto ganglioside G M1 . These results should allow further studies towards elucidating a possible role for the S. typhi enterotoxin in the pathogenesis of typhoid fever.