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Nucleotide sequence of the ugp genes of Escherichia coli K‐12: homology to the maltose system
Author(s) -
Overduin P.,
Boos W.,
Tommassen J.
Publication year - 1988
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1988.tb00088.x
Subject(s) - biology , operon , periplasmic space , escherichia coli , nucleic acid sequence , gene , homology (biology) , maltose binding protein , biochemistry , genetics , nucleotide , open reading frame , peptide sequence , recombinant dna , fusion protein
Summary The nucleotide sequence of the ugp genes of Escherichia Coli K‐12, which encode a phosphate‐limitation inducible uptake system for sn‐glycerol‐3‐phosphate and glycerophosphoryl diesters, was determined. The genetic organization of the operon differed from previously published results. A single promoter, containing a putative pho box, was detected by S1‐nuclease mapping. The promoter is followed by four open reading frames, designated ugpB, A, E and C , which encode a periplasmic binding protein, two hydrophobic membrane proteins and a protein that is likely to couple energy to the transport system, respectively. The sequences of the proteins contain the characteristics of several other binding protein‐dependent transport systems, but they seem to be particularly closely related to the maltose system.