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Epitope mapping with solid‐phase peptides: identification of type‐, subspecies‐, species‐ and genus‐reactive antibody binding domains on the major outer membrane protein of Chlamydia trachomatis
Author(s) -
Conlan J. W.,
Clarke I. N.,
Ward M. E.
Publication year - 1988
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1988.tb00076.x
Subject(s) - epitope , biology , chlamydia trachomatis , serotype , monoclonal antibody , bacterial outer membrane , virology , epitope mapping , antibody , subspecies , microbiology and biotechnology , biochemistry , genetics , gene , escherichia coli , zoology
Summary The major outer membrane protein (MOMP) of Chlamydiatrachomatis carries serovar‐, subspecies‐, species‐ and genus immunodomains, antibodies to which may be protective. We have compared the inferred amino acid sequences for MOMP from different servoars of C. trachomatis and from Chlamydia psittaci to identify the likely locations of these sero‐taxonomic epitopes. Overlapping peptides corresponding to each of these regions were synthesized on a solid phase and probed with monoclonal antibodies (MAbs) of appropriate specificities. We describe the primary structures of the binding sites of MAb to each of these four epitopes on C. trachomatis serovarL1 MOMP