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Molecular cloning, identification and ranscriptional analysis of genes involved in acetate utilization in Neurospora crassa
Author(s) -
Thomas C. M.,
Connerton I. F.,
Fincham J. R. S.
Publication year - 1988
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1988.tb00068.x
Subject(s) - neurospora crassa , biology , cloning (programming) , gene , genetics , identification (biology) , neurospora , crassa , molecular cloning , computational biology , botany , peptide sequence , mutant , computer science , programming language
Summary Four Neurospora crassa genomic clones have been selected as hybridizing much more strongly to labeled mRNA isolated from acetate‐grown mycelium than to mRNA from sucrose‐grown mycelium. Hybridization of restriction fragments with acetate‐specific mRNA or cDNA has been used to delimit the transcribed region(s) of each clone. The transcription of all four clones is strongly induced by transfer of growing mycelium from sucrose to acetate as sole carbon source. In wild‐type mycelium, mRNAs corresponding to the four clones reach maximum levels after four hours of induction. They accumulate more rapidly and reach higher levels in an acetate non‐utilizing mutant, acu‐7 , which has been found to overproduce enzymes of the glyoxylate cycle and to have a partial block in the TCA cycle. Molecular transformation of a Neurosporaacu‐5 mutant and of an Aspergillus nidulans acuE mutant by DNA of clone 2 and clone 1, respectively, strongly suggests that clone 2 codes for acetyl‐coenzyme A synthetase and that clone 1 codes for malate synthase. The transcribed segments of clones 1 and 2each hybridize to corresponding clones from Aspergillusnidulans (R. A. Sandeman and M. J. Hynes, personal communication).