Premium
Transcriptional analysis of the 16S rRNA gene of the rrnD gene set of Streptomyces coelicolor A3(2)
Author(s) -
Baylis H. A.,
Bibb M. J.
Publication year - 1988
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1988.tb00065.x
Subject(s) - streptomyces coelicolor , biology , gene , 16s ribosomal rna , genetics , computational biology , ribosomal rna , mutant
Summary The nucleotide sequence of 2.5 kb of the Streptomyces coelicolor A3(2) rRNA gene set rrnD , extending from upstream of the 16S rRNA gene to the putative 5′ end of the 23S rRNA gene, has been determined (Baylis and Bibb, 1987; this paper). In addition to locating the 5′ end of the 16S rRNA gene, nuclease S 1 mapping identified seven RNA 5′ end‐points upstream of the 16S rRNA gene; four of these were coincident with transcriptional initiation points for S. coelicolor A3(2) RNA poiymerase in vitro and were consequently regarded as in vivo transcription start points for promoters p1 to p4. One end‐point identified by nuclease S 1 mapping localized a putative processing site analogous to those found upstream of 16S rRNA genes in other eubacteria. Sequence motifs similar to those discovered in low G+C Gram‐positive bacteria were found associated with two of the promoters and the processing site. A probable protein coding region was observed upstream of the promoter region.