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The structure of a plasmid of Chlamydia trachomatis believed to be required for growth within mammalian cells
Author(s) -
Comanducci M.,
Ricci S.,
Ratti G.
Publication year - 1988
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1988.tb00060.x
Subject(s) - orfs , biology , plasmid , open reading frame , genetics , chlamydia trachomatis , gene , nucleic acid sequence , start codon , autonomously replicating sequence , peptide sequence , microbiology and biotechnology , origin of replication , virology , base sequence
Summary Sequence analysis of a 7.5 kb DNA plasmid isolated from Chlamydia trachomatis shows 8 open reading frames (ORFs) regularly spaced along most of the sequence. One of these ORFs encodes a 451‐amino‐acid polypeptide highly homologous to the DnaB protein of Escherichia coli. A region between ORFs 6 and 7 contains a cluster of alternating ATs and a 22 bp sequence tandemly repeated 4 times, suggesting a replication control region. Several ORFs correspond to plasmid‐specific polypeptides that have been described. Codons ending with A or T are more frequent, as might be expected from the high A/T content (64%) of the plasmid, and codon usage is similar to that of the C. trachomatis chromosomal gene, omp1L2.