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The expression of mutant pilins in Pseudomonas aeruginosa : fifth position glutamate affects pilin methylation
Author(s) -
Pasloske B. L.,
Paranchych W.
Publication year - 1988
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1988.tb00055.x
Subject(s) - pilin , biology , methylation , mutant , amino acid , pseudomonas aeruginosa , pilus , peptide sequence , biochemistry , peptide , proteolysis , microbiology and biotechnology , genetics , gene , bacteria , escherichia coli , enzyme
Summary The expression within Pseudomonas aeruginosa PAO1 of three mutant pilin genes from P. aeruginosa PAK was studied to determine their effects on pilin stability, translocation into the membrane, leader peptide removal, and methylation of the mature N‐terminal phenylalanine. The results revealed that a deletion of 4 or 8 amino acids within the immediate N‐terminus of pilin had deleterious effects upon leader peptide cleavage. In addition, while the 4‐amino‐acid deletion did not affect pilin partitioning into the membrane, the 8‐amino‐acid deletion decreased the amount of pilin found within the membrane fraction. Of considerable interest was the finding that the mutation within the mature pitin of the glutamate at position 5 to a lysine did not prevent leader peptide removal but did inhibit the methylation of the N‐terminal phenylalanine.