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Nucleotide sequence of the mannitol (mtl) operon in Escherichia coli
Author(s) -
Davis T.,
Yamada M.,
Elgort M.,
Saier M. H.
Publication year - 1988
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1988.tb00045.x
Subject(s) - biology , operon , nucleic acid sequence , gene , repressor , microbiology and biotechnology , palindromic sequence , genetics , escherichia coli , gene expression , crispr , palindrome
Summary The nucleotide sequence of the known portions of the mannitol operon in Escherichia coli (mtlOPAD) has been determined. Both the operator‐promoter region and the intercistronic region between the mtlA and mtID genes (encoding the mannitol‐specific Enzyme II of the phosphotransferase system and mannitol–1‐ phosphate dehydrogenase, respectively) show parallels with corresponding regions of the glucitol (gut) operon, but neither the mtlA nor the mtlD gene products show obvious homology with the corresponding gene products of the glucitol operon. Five potential cyclic AMP receptor protein binding sites were identified in the mtlOP region, all showing near identity with the consensus sequence. Four regions of dyad symmetry (four to seven bases in length), serving as potential repressor binding sites, overlap with the potential cyclic AMP receptor protein binding sites. Repetitive extragenic pallndromic (REP) sequences, forming stem‐loop structures in the intercistronic region between mtlA and mtlD and following the mtlD gene were identified. Probable terminator sequences were not found in any of these three regulatory regions. Mannitol–1‐phosphate dehydrogenase exhibits two overlapping, potential NAD + binding sites near the W‐terminus of the protein. Computer techniques were used to analyse the mtlD gene and its product.