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Organization and regulation of the Corynebacterium glutamicum hom‐thrB and thrC loci
Author(s) -
Follettie M. T.,
Shin H. K.,
Sinskey A. J.
Publication year - 1988
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1988.tb00006.x
Subject(s) - corynebacterium glutamicum , biology , operon , gene , threonine , genetics , transcription (linguistics) , locus (genetics) , homoserine , microbiology and biotechnology , serine , phosphorylation , quorum sensing , escherichia coli , linguistics , philosophy , virulence
Summary The genes encoding the three terminal enzymes in the threonine biosynthetic pathway, homoserine dehydrogenasa ( hom ), homoserine kinase ( thrB ) and threonine synthase ( thrC ) have been isolated from Corynebacterium glutamicum. The C. glutamicum hom and thrB genes were subcloned on a 3.6 kb Sal l‐generated chromosomal fragment. The C. glutamicum thrC gene was shown not to be linked to the hom‐thrB locus. L‐methionine represses the cloned homoserine dehydrogenase and homoserine kinase similar to that of the chromosomally encoded hom and thrB gene products. Northern hybridization analysis demonstrates that this repression is mediated at the level of transcription and that hom‐thrB represents an operon in C. glutamicum.