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The ParD ‐ mutant of Escherichia coli also carries A am mutation. The complete sequence of gyrA
Author(s) -
Hussain K.,
Elliott E. J.,
Salmond G. P. C.
Publication year - 1987
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1987.tb01932.x
Subject(s) - biology , genetics , mutant , gene , mutation , dna gyrase , point mutation , escherichia coli , phenotype , microbiology and biotechnology
Summary The phenotype of a recently‐described mutant (OVG), conditionally defective in chromosome partitioning and septal positioning, was originally thought to be due to a new gene ( parD ) mapping at 88.4 min. We have now shown that, in addition to the parD mutation, OV6 carries a gyrA am mutation and that this mutation is probably responsible for the gross phenotype of the mutant. We have cloned the gyrA gene, identified the GyrA protein, sequenced the gyrA gene and flanking genes, cloned and sequenced the gyrA am , mutation, and identified its truncated product, in addition, we have identified the transcriptional start point of the gyrA gene. The E. coli GyrA protein has extensive homologies with Gyrase proteins of other organisms and weak sequence homologies with some eukaryotic cytoskeletal proteins.