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Exotoxin A of Pseudomonas aeruginosa: evidence that Domain I functions In receptor binding
Author(s) -
GuidiRontani C.,
Collier R. J.
Publication year - 1987
Publication title -
molecular microbiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.857
H-Index - 247
eISSN - 1365-2958
pISSN - 0950-382X
DOI - 10.1111/j.1365-2958.1987.tb00528.x
Subject(s) - pseudomonas exotoxin , biology , mutant , exotoxin , pseudomonas aeruginosa , escherichia coli , gene , cytotoxicity , receptor , binding domain , microbiology and biotechnology , function (biology) , cytotoxic t cell , toxin , biochemistry , binding site , bacteria , genetics , in vitro
We have constructed defined deletions in the structural gene of Pseudomonas aeruginosa exotoxin A (ETA) In order to probe the function of Domain t of this protein. Three forms of the gene containing specific deletions were expressed in a strain of Escherichia coli K12 with lesions in the htpR and ion genes; extracts containing the gene products were tested for ADP‐ribosylation activity, cytotoxicity, and ability to protect sensitive cells from the cytotoxic action of authentic ETA. Two of the mutant ETAs gave concentration‐dependent protection against authentic ETA, and protection correlated with the presence of the bulk of Domain I. The results support the notion that Domain I functions in binding the toxin to specific cell‐surface receptors.