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Scanning the European corn borer ( Ostrinia spp.) genome for adaptive divergence between host‐affiliated sibling species
Author(s) -
MIDAMEGBE AFIWA,
VITALIS RENAUD,
MALAUSA THIBAUT,
DELAVA ÉMILIE,
CROSARTEIL SANDRINE,
STREIFF RÉJANE
Publication year - 2011
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.2011.05035.x
Subject(s) - ostrinia , biology , european corn borer , genetics , genome , amplified fragment length polymorphism , sympatric speciation , population , evolutionary biology , pest analysis , pyralidae , botany , gene , genetic diversity , demography , sociology
It has recently been shown that the European corn borer, a major pest of maize crops, is actually composed of two genetically differentiated and reproductively isolated taxa, which are found in sympatry over a wide geographical range in Eurasia. Each taxon is adapted to specific host plants: Ostrinia nubilalis feeds mainly on maize, while O. scapulalis feeds mainly on hop or mugwort. Here, we present a genome scan approach as a first step towards an integrated molecular analysis of the adaptive genomic divergence between O. nubilalis and O. scapulalis . We analysed 609 AFLP marker loci in replicate samples of sympatric populations of Ostrinia spp . collected on maize, hop and mugwort, in France. Using two genome scan methods based on the analysis of population differentiation, we found a set of 28 outlier loci that departed from the neutral expectation in one or the other method (of which a subset of 14 loci were common to both methods), which showed a significantly increased differentiation between O . nubilalis and O. scapulalis , when compared to the rest of the genome. A subset of 12 outlier loci were sequenced, of which 7 were successfully re‐amplified as target candidate loci. Three of these showed homology with annotated lepidopteran sequences from public nucleotide databases.