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Heterologous hybridization to a complementary DNA microarray reveals the effect of thermal acclimation in the endothermic bluefin tuna ( Thunnus orientalis )
Author(s) -
CASTILHO PEDRO C.,
BUCKLEY BRADLEY A.,
SOMERO GEORGE,
BLOCK BARBARA A.
Publication year - 2009
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.2009.04174.x
Subject(s) - biology , thunnus , tuna , acclimatization , fishery , heterologous , zoology , ecology , fish <actinopterygii> , genetics , gene
The temperature stress that pelagic fishes experience can induce physiological and behavioural changes that leave a signature in gene expression profiles. We used a functional genomics approach to identify genes that were up‐ or down‐regulated following thermal stress in the Pacific bluefin tuna. Following the acclimation period, 113, 81 and 196 genes were found to be differentially expressed between the control (20 °C) and cold (15°) treatment groups, in ventricle, red muscle and white muscle, respectively. The genes whose expression levels were responsive to thermal acclimation varied according to muscle fibre type, perhaps reflecting the tissue‐specific degrees of endothermy characteristic of this species.