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Genomics of hybrid poplar ( Populus trichocarpa × deltoides ) interacting with forest tent caterpillars ( Malacosoma disstria ): normalized and full‐length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insect‐induced defences in poplar
Author(s) -
RALPH STEVEN,
ODDY CLAIRE,
COOPER DAWN,
YUEH HESTHER,
JANCSIK SHARON,
KOLOSOVA NATALIA,
PHILIPPE RYAN N.,
AESCHLIMAN DANA,
WHITE RICK,
HUBER DEZENE,
RITLAND CAROL E.,
BENOIT FRANÇOIS,
RIGBY TRACEY,
NANTEL ANDRÉ,
BUTTERFIELD YARON S. N.,
KIRKPATRICK ROBERT,
CHUN ELIZABETH,
LIU JERRY,
PALMQUIST DIANA,
WYNHOVEN BRIAN,
STOTT JEFFREY,
YANG GEORGE,
BARBER SARAH,
HOLT ROBERT A.,
SIDDIQUI ASIM,
JONES STEVEN J. M.,
MARRA MARCO A.,
ELLIS BRIAN E.,
DOUGLAS CARL J.,
RITLAND KERMIT,
BOHLMANN JÖRG
Publication year - 2006
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.2006.02824.x
Subject(s) - biology , expressed sequence tag , complementary dna , functional genomics , cdna library , genetics , gene , populus trichocarpa , gene expression profiling , methyl jasmonate , genome , botany , genomics , gene expression
Abstract As part of a genomics strategy to characterize inducible defences against insect herbivory in poplar, we developed a comprehensive suite of functional genomics resources including cDNA libraries, expressed sequence tags (ESTs) and a cDNA microarray platform. These resources are designed to complement the existing poplar genome sequence and poplar ( Populus spp.) ESTs by focusing on herbivore‐ and elicitor‐treated tissues and incorporating normalization methods to capture rare transcripts. From a set of 15 standard, normalized or full‐length cDNA libraries, we generated 139 007 3′‐ or 5′‐end sequenced ESTs, representing more than one‐third of the c. 385 000 publicly available Populus ESTs. Clustering and assembly of 107 519 3′‐end ESTs resulted in 14 451 contigs and 20 560 singletons, altogether representing 35 011 putative unique transcripts, or potentially more than three‐quarters of the predicted c. 45 000 genes in the poplar genome. Using this EST resource, we developed a cDNA microarray containing 15 496 unique genes, which was utilized to monitor gene expression in poplar leaves in response to herbivory by forest tent caterpillars ( Malacosoma disstria ). After 24 h of feeding, 1191 genes were classified as up‐regulated, compared to only 537 down‐regulated. Functional classification of this induced gene set revealed genes with roles in plant defence (e.g. endochitinases, Kunitz protease inhibitors), octadecanoid and ethylene signalling (e.g. lipoxygenase, allene oxide synthase, 1‐aminocyclopropane‐1‐carboxylate oxidase), transport (e.g. ABC proteins, calreticulin), secondary metabolism [e.g. polyphenol oxidase, isoflavone reductase, (–)‐germacrene D synthase] and transcriptional regulation [e.g. leucine‐rich repeat transmembrane kinase, several transcription factor classes (zinc finger C3H type, AP2/EREBP, WRKY, bHLH)]. This study provides the first genome‐scale approach to characterize insect‐induced defences in a woody perennial providing a solid platform for functional investigation of plant–insect interactions in poplar.