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PCR‐based identification of wheat genomes
Author(s) -
SALLARES R.,
ALLABY R. G.,
BROWN T. A.
Publication year - 1995
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.1995.tb00246.x
Subject(s) - biology , genome , locus (genetics) , genetics , primer (cosmetics) , ploidy , dna , evolutionary biology , gene , computational biology , chemistry , organic chemistry
We describe a PCR system that distinguishes the A, B and D genomes in wheat DNA extracts. PCRs were directed at the ‘non‐transcribed spacer’ regions of the rDNA loci. The spacers within the D genome locus have a 71‐bp insertion that is absent from the corresponding A and B loci PCR product sizes therefore enable D‐ and D+ genomes to be distinguished. The A and B genomes can be differentiated by PCR with an internal primer which does not anneal to A genome sequences. This work is relevant to the ancient ecology of wheat, as it is often difficult to determine ploidy level from morphological examination of archaeobotanical remains.