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Differentiation of the endemic New Zealand greenheaded and brownheaded leafroller moths by restriction fragment length variation in the ribosomal gene complex
Author(s) -
SIN F. Y. T.,
SUCKLING D. M.,
MARSHALL J. W.
Publication year - 1995
Publication title -
molecular ecology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.619
H-Index - 225
eISSN - 1365-294X
pISSN - 0962-1083
DOI - 10.1111/j.1365-294x.1995.tb00215.x
Subject(s) - biology , bglii , genetics , ribosomal rna , restriction enzyme , restriction fragment length polymorphism , gene , ribosomal dna , microbiology and biotechnology , evolutionary biology , hindiii , polymerase chain reaction , phylogenetics
This study identified markers in the ribosomal RNA gene complex (rDNA) and evaluated their utility in the differentiation of four endemic New Zealand species of leafroller moth, Planotortrix excessana (Walker), P. octo (Dugdale), Ctenopseustis herana (Felder and Rogenhofer) and C. obliquana (Walker). Pooled genomic DNA from laboratory‐reared specimens of C. herana and C. obliquana could be distinguished with a single restriction enzyme (e.g. ClaI ), while P. excessana and P. octo could be satisfactorily distinguished using double digestions with either ClaI/PvuII or ClaI/BglII . It is concluded mat these markers may be used to identify moths from wild populations.